Role of Fas and Treg cells in fracture healing as characterized in the Fas‐deficient (lpr) mouse model of lupus
Date
2014-05-19
Authors
Al‐Sebaei, Maisa O.
Daukss, Dana M.
Belkina, Anna
Kakar, Sanjeev
Wigner, Nathan A.
Cusher, Daniel
Graves, Dana
Einhorn, Thomas
Morgan, Elise
Gerstenfeld, Louis C.
Version
OA Version
Citation
Maisa O Al-Sebaei, Dana M Daukss, Anna C Belkina, Sanjeev Kakar, Nathan A Wigner, Daniel Cusher, Dana Graves, Thomas Einhorn, Elise Morgan, Louis C Gerstenfeld (2014), Role of Fas and Treg Cells in Fracture Healing as Characterized in the Fas-Deficient (lpr) Mouse Model of Lupus. J Bone Miner Res, 29: 1478–1491. doi:10.1002/jbmr.2169
Abstract
Previous studies showed that loss of tumor necrosis factora (TNFa) signaling delayed fracture healing by delaying chondrocyte apoptosis and cartilage resorption. Mechanistic studies showed that TNFa induced Fas expression within chondrocytes; however, the degree to which chondrocyte apoptosis ismediated by TNFa alone or dependent on the induction of Fas is unclear. This questionwas addressed by assessing fracture healing in Fas‐deficient B6.MRL/Faslpr/Jmice. Loss of Fas delayed cartilage resorption but also lowered bone fraction in the calluses. The reduced bone fraction was related to elevated rates of coupled bone turnover in the B6.MRL/Faslpr/J calluses, as evidenced by higher osteoclast numbers and increased osteogenesis. Analysis of the apoptoticmarker caspase 3 showed fewer positive chondrocytes and osteoclasts in calluses of B6.MRL/Faslpr/J mice. To determine if an active autoimmune state contributed to increased bone turnover, the levels of activated T cells and Treg cellswere assessed. B6.MRL/Faslpr/J mice had elevated Treg cells in both spleens and bones of B6.MRL/Faslpr/J but decreased percentage of activated T cells in bone tissues. Fracture led to 30% to 60% systemic increase in Treg cells in bothwild‐type and B6.MRL/Faslpr/J bone tissues during the period of cartilage formation and resorption but either decreased (wild type) or left unchanged (B6.MRL/Faslpr/J) the numbers of activated T cells in bone. These results show that an active autoimmune state is inhibited during the period of cartilage resorption and suggest that iTreg cells play a functional role in this process. These data show that loss of Fas activity specifically in chondrocytes prolonged the life span of chondrocytes and that Fas synergized with TNFa signaling tomediate chondrocyte apoptosis. Conversely, loss of Fas systemically led to increased osteoclast numbers during later periods of fracture healing and increased osteogenesis. These findings suggest that retention of viable chondrocytes locally inhibits osteoclast activity or matrix proteolysis during cartilage resorption.
Description
License
© 2014 The Authors. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.